Custom Polyclonal Antibody

 

Custom Polyclonal Antibody Production

Custom polyclonal antibody production services are paid company services that will develop a polyclonal antibody for you.

 

Custom Polyclonal Development Services

Often you look for your antibody of interest and it is not in any antibody catalogues. If you are looking to make an antibody for either immunoprecipitation, total protein western blot analysis or similar methods then you need custom polyclonals to be developed.

 

Steps in Custom Polyclonal Development

The company you pick can produce custom polyclonal antibodies for you using an antigen you provide (this can either be purified protein, purified recombinant protein or peptide), or if you do not have these then the company should be able to design and synthesized a peptide antigen that will be guaranteed immunogenic.

Animals are put on an immunization protocol and screened to check their bleeds for antibody production against your protein. Your antibody can be then purified.

Rabbits can be terminated, or exsanguinated to harvest additional serum for antibody production. Batches of animals can extend the production of your polyclonal antibody for many years, allowing you to obtain as much antibody anti-serum for use in your research.

 

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