Monoclonal Antibody Monoklonalt antistof

Looking for Monoclonal Antibodies? Looking for monoklonale antistoffer?

Monoclonal Antibodies for Western Blot Analysis : Monoklonale antistoffer til Western Blot Analyse:

A Monoclonal antibody provides higher specificity, purity and consistency than polyclonal antibodies, resulting in lower backgrounds in western blot results. Et monoklonalt antistof giver højere specificitet, renhed og konsistens end polyklonale antistoffer, hvilket resulterer i lavere baggrunde i de vestlige skamplet resultater.

Monoclonal Antibody Services : Monoklonalt antistof Services:

Looking to create specific antibodies for your protein of interest? Looking for at oprette specifikke antistoffer for din protein af interesse? There are many custom services which can create a monoclonal antibody for you in several weeks to months. Der er mange specielle tjenester, der kan skabe et monoklonalt antistof til dig i flere uger til måneder.

There are several ways of making monoclonal antibodies: Der er flere måder at gøre monoklonale antistoffer:

How Much Antigen or Protein do I need for Monoclonal Antibody Development? Hvor meget Antigen eller protein har jeg brug for monoklonalt antistof Udvikling?

For Peptides as Antigens: For peptider som antigener:

- 10 mg is generally enough -- 10 mg er generelt nok

- less peptide may be needed if your peptide is highly purified -- Mindre peptid kan være nødvendige, hvis din peptid er meget renset

- if you need affinity purification of an antibody, around 15 mg of peptide may be needed in dry form. -- Hvis du har brug affinitet rensning af et antistof, omkring 15 mg peptid kan være nødvendig i tør form.

For Protein Antigens: For Protein antigener:

- 2-5 mg's of protein antigen at a concentration of 0.5-1 mg/mL are usually needed. -- 2-5 mg's protein-antigen i en koncentration på 0.5-1 mg / ml er normalt nødvendigt.

- indicate the buffer used and the protein concentration. -- Anføre buffer anvendes, og protein koncentration.

-  Protein should be 3 - 5 mg at a concentration of 0.5 mg/ml or more. -- Protein bør være 3 - 5 mg i en koncentration på 0,5 mg / ml eller derover.

- for antibody affinity purification, 5 mg of soluble protein is needed to prepare an immunoaffinity column. -- For antistof affinitet rensning, 5 mg af opløseligt protein er nødvendig for at forberede en immunoaffinity kolonne.

Characteristics of the Monoclonal Antibody: Karakteristik af det monoklonale antistof:

• Specific - due to their nature of originating from one clone specificity is the same for every antibody Specifikke - på grund af deres karakter, der stammer fra en klon specificitet er den samme for hvert antistof
• Isotype - all antibodies (and monoclonal antibody batches) are identical giving reproducibility Isotype - alle antistoffer (og monoklonalt antistof partier) er identiske giver reproducerbarhed
• Affinity for antigen - monoclonal antibodies Affinitet for antigen - monoklonale antistoffer
• Stability Stabilitet

Applications of Monoclonal Antibodies : Anvendelser af monoklonale antistoffer:

Benefits of the Monoclonal Antibody: Fordele ved det monoklonale antistof:

Monoclonal antibodies are excellent as the primary antibody in an assay due to their great specificity. Monoklonale antistoffer er fremragende som det primære antistof i en analyse på grund af deres store specificitet. Monoclonals recognize only one epitope of the antigen and are highly specific to that particular antigen, and thus will usually give substantially less background staining than polyclonal antibodies. Monoklonale antistoffer genkender kun en epitop af antigenet, og de er meget specifikke for denne specifikke antigen, og dermed vil normalt give væsentligt mindre baggrundsviden farvning end polyklonale antistoffer. . When compared to the heterogeneity of polyclonal antibodies, the homogeneity of monoclonal antibodies is very high. I forhold til den uensartethed polyklonale antistoffer, homogenitet af monoklonale antistoffer er meget høj. If experimental conditions are kept constant, using monoclonal antibodies will make results consitently reproducible between experimental replicates due to the clonal nature of monoclonal antibodies. Hvis eksperimentelle betingelser, der holdes konstant, ved hjælp af monoklonale antistoffer vil gøre resultaterne consitently reproducerbare mellem eksperimentelle replikater på grund af den klonede karakter af monoklonale antistoffer.   Their specificity also makes them extremely efficient for the binding of antigen within a mixture of related molecules, such as in the case of affinity purification. Deres særegenhed også gør dem ekstremt effektive for binding af antigen i en blanding af beslægtede molekyler, sådan som det er tilfældet med affinitet rensning.