antistoffer Antibody Station
Polyclonal Antibodies Polyklonale Antistoffer

Polyclonal Antibody Polyklonale Antibody

Polyclonal antibodies are antibodies that are derived from many different cells or cell lines, similar to the mixture of antibodies found in sera. Polyklonale antistoffer er antistoffer, der stammer fra mange forskellige celler eller cellelinjer, der ligner en blanding af antistoffer i sera. Polyclonal antibodies are therefore a mixture of many different specificities. Polyklonale antistoffer er derfor en blanding af mange forskellige specificiteter. This is in contrast to monoclonal antibodies which are derived from one clone. Dette er i modsætning til monoklonale antistoffer, som er afledt af en klon.

When researchers purchase a polyclonal antibody preparation, this is usually a mixture of antibodies of many different specificities to the same antigen. Når forskere købe et polyklonalt antistof forberedelse, er det normalt en blanding af antistoffer af mange forskellige specificiteter til samme antigen.

Polyclonal antibodies are produced by immunizing a rabbit, mouse, goat, chicken, or other animal. Polyklonale antistoffer er produceret af immuniserende en kanin, mus, geder, høns, eller andre dyr. The antigen (that you want to generate an antibody against) is injected into the animal along with a suitable adjuvant. Antigenet (som du ønsker at generere et antistof imod) sprøjtes ind i dyret sammen med en passende adjuvans. This immunization of the animal results in the production of immunoglobulins specific to that antige, of which IgG (immunoglobulin G) is the most desirable. Denne immunisering af dyret resulterer i produktion af immunoglobuliner er specifikt for den pågældende antige, hvoraf IgG (immunoglobulin G) er den mest ønskelige. This is due to the binding properties of IgG, its high concentrations in serum, and because it is the easiest class of immunoglobulin to handle in the laboratory. Dette skyldes den bindende egenskaber af IgG, dets høje koncentrationer i serum, og fordi det er den letteste klasse af immunglobulin til at håndtere i laboratoriet. The animal sera can be used directly, however it is usually purified to yield purer polyclonal antibody preparations. De animalske sera kan bruges direkte, dog er det som regel renses for at give renere polyklonale antistof præparater.