Monoclonal Antibody Monoklonsko antitijelo

Looking for Monoclonal Antibodies? Tražite monoklonska protutijela?

Monoclonal Antibodies for Western Blot Analysis : Monoklonska protutijela za Western izbrisat Analiza:

A Monoclonal antibody provides higher specificity, purity and consistency than polyclonal antibodies, resulting in lower backgrounds in western blot results. A monoklonsko antitijelo pruža veći specifičnost, čistoća i konzistentnost nego polyclonal protutijela, što je rezultiralo nižim pozadina u zapadnoj izbrisati rezultate.

Monoclonal Antibody Services : Monoklonsko antitijelo usluge:

Looking to create specific antibodies for your protein of interest? Potražnja za stvaranje specifičnih protutijela za Vaše protein od interesa? There are many custom services which can create a monoclonal antibody for you in several weeks to months. Postoje mnoge korisničke usluge koje mogu stvoriti monoklonsko antitijelo za Vas u nekoliko tjedana do mjeseci.

There are several ways of making monoclonal antibodies: Postoji nekoliko načina izrade monoklonska protutijela:

How Much Antigen or Protein do I need for Monoclonal Antibody Development? Koliko antigen ili protein trebam za monoklonsko antitijelo za razvoj?

For Peptides as Antigens: Za Fotokromizam i Termokromizam kao antigeni:

- 10 mg is generally enough -- 10 mg je dovoljno općenito

- less peptide may be needed if your peptide is highly purified -- Manje peptida svibanj biti potreban ako je vaš peptida je visoko očistili

- if you need affinity purification of an antibody, around 15 mg of peptide may be needed in dry form. -- Ako je potrebno afinitet pročišćavanje jedan antitijela, oko 15 mg, peptida svibanj biti potrebne u obliku suhe.

For Protein Antigens: Za protein antigeni:

- 2-5 mg's of protein antigen at a concentration of 0.5-1 mg/mL are usually needed. -- 2-5 mg's of protein antigen u koncentraciji 0.5-1 mg / mL su obično potrebne.

- indicate the buffer used and the protein concentration. -- Ukazati tampon koristiti i protein concentration.

-  Protein should be 3 - 5 mg at a concentration of 0.5 mg/ml or more. -- Protein bi trebao biti 3 do 5 mg u koncentraciji od 0,5 mg / ml ili više.

- for antibody affinity purification, 5 mg of soluble protein is needed to prepare an immunoaffinity column. -- Antitijela afinitet za pročišćavanje, 5 mg protein, topljiv je potrebno pripremiti jedan immunoaffinity column.

Characteristics of the Monoclonal Antibody: Karakteristike je monoklonsko antitijelo:

• Specific - due to their nature of originating from one clone specificity is the same for every antibody Specifične - zbog njihove prirode podrijetlom iz jedne klonom specifičnost je isti za svaki antitijela
• Isotype - all antibodies (and monoclonal antibody batches) are identical giving reproducibility Isotype - sve protutijela (i monoklonska protutijela batches) su identične davanje reproducibilnost
• Affinity for antigen - monoclonal antibodies Afinitet za antigen - monoklonska protutijela
• Stability Stabilnosti

Applications of Monoclonal Antibodies : Prijave za monoklonska protutijela:

Benefits of the Monoclonal Antibody: Prednosti su monoklonsko antitijelo:

Monoclonal antibodies are excellent as the primary antibody in an assay due to their great specificity. Monoklonska protutijela su odlične kao primarna antitijela u jedan esej zbog njihove velike specifičnosti. Monoclonals recognize only one epitope of the antigen and are highly specific to that particular antigen, and thus will usually give substantially less background staining than polyclonal antibodies. Monoclonals priznaju samo jedan epitope na antigen i vrlo su specifični za taj određeni antigen, a time će obično dati znatno manje pozadini staining od polyclonal protutijela. . When compared to the heterogeneity of polyclonal antibodies, the homogeneity of monoclonal antibodies is very high. Kad je u odnosu na heterogeneity of polyclonal protutijela, homogeneity, monoklonska protutijela je vrlo visok. If experimental conditions are kept constant, using monoclonal antibodies will make results consitently reproducible between experimental replicates due to the clonal nature of monoclonal antibodies. Ako se eksperimentalni uvjeti su držali stalan, koriste monoklonska protutijela će rezultate consitently reproducible između eksperimentalne odgovor s obzirom na prirodu clonal monoklonska protutijela.   Their specificity also makes them extremely efficient for the binding of antigen within a mixture of related molecules, such as in the case of affinity purification. Njihova specifičnost također ih čini izuzetno efikasnom za obvezujuća za antigen u roku od mješavine srodnih molekula, kao što je u slučaju afinitet za pročišćavanje.